Retention of both primary and secondary infecting parasites following secondary challenge despite protection from disease pathology.

Abstract

<p>The graph plots the number of persistent parasites present in sites of primary and secondary <i>Leishmania</i> infections >10 weeks post secondary challenge as assessed by limiting dilution analysis in unselective (white bar), nourseothricin-containing (gray bars; resistance mediated by <i>SAT</i> marker) or phleomycin-containing (black bars; resistance mediated by <i>PHLEO</i> marker) as described in the methods. The number of parasites in the primary infection site (LmjF-LUC-SAT inocula) is displayed in the top graph, and the number of parasites in secondary infection site (LmjF-PHLEO inocula) foot is displayed in the bottom graph. The numbers between the two graphs represent the mouse identification number (experiment number-mouse number). “Avg.” represents the mean for all mice.</p