<p>One copy of the sequence [(AGSGAG)<sub>6</sub>ASEYDYDDDSDDDDEWD]<sub>2</sub> (A). (AGSGAG)<sub>n</sub> is the silk fibroin repetitive domain from <i>B</i>. <i>mori</i>. The calcium-binding protein sequence from the shell nacreous matrix protein MSI60 is marked by a black line. The vector used for the transgenic experiment (B). ITR, inverted terminal repeats of PB transposon; FL, fibroin light chain; FLSP, signal peptide of fibroin light chain; CABP, calcium-binding protein; A3, <i>Bombyx mori</i> A3 cytoplasmic actin gene; EGFP, enhanced green fluorescence protein; SV40, 3’-untranslated sequences.</p