<p>(A–C) Whole cell lysates of ACEA-treated HepG2 cells and AML12 cells and livers of ACEA-treated intact mice were harvested for western blot analysis. (D–E) AML12 cells and rat primary hepatocytes were treated with ACEA (10 μM) for the indicated time periods. Culture media were collected for FGF21 secretion analysis. (F) Mice were treated with ACEA (10 mg/kg) for the indicated number of days. Serum was obtained for FGF21 secretion analysis. All data are the means ± standard errors of at least three independent experiments. **p < 0.01; ***p < 0.001 by one-way ANOVA.</p

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