LncRNA-HOTAIR rs920778 SNP T variant allele exhibits enhanced enhancer activity and increases HOTAIR expression.

Abstract

<p><b>(A)</b> Enhancer activity are presented as fold increase relative to negative control (NC, empty vector PGL3). “**” indicates <i>P</i><0.01. Individuals with the risk allele T at rs920778 in both 38 normal cervical tissues <b>(B)</b> and 43 cervical cancer tissues <b>(C)</b> is significantly associated with increased HOTAIR expression than those with the CC genotypes. Expression levels of HOTAIR are detected by RT-PCR in cervical cancer tissues grouped to three genotypes (rs920778TT, rs920778CT or rs920778CC). “*” indicates <i>P</i><0.05. <b>(D)</b> RT-PCR analysis of HOTAIR expression levels in cervical cancer cell lines (SiHa, Hela and Caski) compared with breast cancer cell MCF-7 and human embryo kidney epithelial cell 293T. <b>(E)</b> The HOTAIR expression level was analyzed by RT-PCR in 91 cervical cancer tissue samples. HOTAIR expression levels was significantly upregulated in cervical cancer tissues compared with their matched non-tumor cervical cancer tissues (<i>P</i><0.05). T, tumor; N, no-tumor.</p