Immobilization of the ARG1 fusions to the cell outer membrane.

Abstract

<p>(A) SDS-PAGE and Western-Blot analysis of the human ARG1 fusions on the outer membrane. Upper panel is the SDS-PAGE analysis and the lower panel is the western-blot analysis. M: protein ladder; T: total cell proteins; OM: outer membrane proteins. InaK-N/ARG1; ssTorA-InaK-N/ARG1; K₆-InaK-N/ARG1; E₆-InaK-N/ARG1; D₆-InaK-N/ARG1; and ssMalE-InaK-N/ARG1 presented the recombinant strain expressing these fusion proteins, respectively; Control presented the recombinant strain containing empty pET23a-T. (B) Enzyme activity and proteinase K accessibility of the InaK-N/ARG1s. Proteinase K-: recombinant strain was untreated with proteinase K; Proteinase K+: recombinant strain was treated with proteinase K. K6-InaK-N/ARG1; E6-InaK-N/ARG1; D6-InaK-N/ARG1; ssMalE-InaK-N/ARG1; K6-InaK-N/ARG1 presented the recombinant strains expressing these fusion proteins, respectively.</p