Constructs in the study.
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Abstract
<p>(A) Different N-terminal fusions of InaK-N. (B) Different InaK-Ns fused with C-terminal human ARG1. (C) Schematic diagram of the cloning method for making the constructs. (D) <i>InaK-Ns</i> PCR products. Lanes 1–6 are PCR products for <i>InaK-N</i>, <i>ssMalE</i>-<i>InaK-N</i>, <i>ssTorA</i>-<i>InaK-N</i>, <i>D</i><sub><i>6</i></sub>-<i>InaK-N</i>, <i>E</i><sub><i>6</i></sub>-<i>InaK-N</i>, and <i>K</i><sub><i>6</i></sub>-<i>InaK-N</i>. (E) PCR products for <i>InaK-N/ARG1s</i>. Lane 1–8 <i>InaK-N</i>, <i>human ARG1</i>, <i>InaK-N/ARG1</i>, <i>ssMalE</i>-<i>InaK/ARG1</i>, <i>ssTorA</i>-<i>InaK-N/ARG1</i>, <i>D</i><sub><i>6</i></sub>-<i>InaK-N/ARG1</i>, <i>E</i><sub><i>6</i></sub>-<i>InaK-N/ARG1</i>, and <i>K</i><sub><i>6</i></sub>-<i>InaK-N/ARG1</i>. M: DNA Ladder.</p
