Fluorescence microscope assay of InaK-N/ARG1s.

Abstract

<p>Surface fluorescence of the cells harboring various InaK-N/ARG1s under a fluorescence microscope with the excitation laser of 488nm, and the EGFP detection channel being used. A-G indicated cells containing empty pET23a-T vector; pET23a-InaK-N/ARG1 vector; pET23a-ssMalE-InaK-N/ARG1 vector; pET23a-ssTorA-InaK-N/ARG1 vector; pET23a-D₆-InaK-N/ARG1 vector; pET23a-E₆-InaK-N/ARG1 vector; and pET23a-K₆-InaK-N/ARG1 vector, respectively. 1: detecting of FITC signal, 2: the bright field, 3: the merge of FITC signal and bright field.</p