Characterization of the cell surface-immobilized InaK-N-E/ARG1s.
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Abstract
<p>(A) Relative activity of the cells bearing K<sub>6</sub>-InaK-N/ARG1 at different pH values. (B) Relative activity of the cells bearing K<sub>6</sub>-InaK-N/ARG1 at different temperatures. (C) Operational stability of the cells bearing K<sub>6</sub>-InaK-N/ARG1. The maximum activity was normalized to 100% in all of the assays. (D) MC trace analysis of the hydrolysis of molecular standard L-Orn. (E) MC trace analysis of the hydrolysis of molecular standard L-Arg.(F) MC trace analysis of the hydrolysis of L-Arg catalyzed by the cells bearing K<sub>6</sub>-InaK-N/ARG1.</p