Flow cytometry assay for InaK-Ns and InaK-N/ARG1s.
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Abstract
<p>Cells containing different vectors labeled with Ddylight649-conjugated antibody against the HA epitope tag were analyzed by flow cytometry. The excitation laser was 638nm, and the emission filter was 660/20 BP. A-M indicated cells containing the pET23a-T empty vector; pET23a-InaK-N vector; pET23a <i>-</i>ssMalE-InaK-N vector; pET23a-ssTorA-InaK-N vector; pET23a-D<sub>6</sub>-InaK-N vector; pET23a-E<sub>6</sub>-InaK-N vector; pET23a-K<sub>6</sub>-InaK-N vector; pET23a-InaK-N/ARG1 vector; pET23a-ssMalE-InaK-N/ARG vector; pET23a-ssTorA-InaK-N/ARG1 vector; pET23a-D<sub>6</sub>-InaK-N/ARG1 vector; pET23a-E<sub>6</sub>-InaK-N/ARG1 vector; and pET23a-K<sub>6</sub>-InaK-N/ARG1 vector, respectively.</p
