Enrichment of H3K4me3 and H3K27me3 at promoter regions of p53 and selenbp1 gene.

Abstract

<p>ChIP assays were performed in 28°C, 18°C /5d and 18°C /30d samples with H3K4me3, H3K27me3 and IgG antibodies. Primers specific for the promoters of <i>p53</i> (A) and <i>selenbp1</i> (B) were used for quantitative PCR of ChIP DNAs. Relative quantitation was carried out by the comparative threshold cycle (CT) method. Statistical analysis was performed using GraphPad Prism 5 software. The Student <i>t</i> test was used on measurements of enrichment from 18°C /5d, 18°C /30d and 28°C samples from 3 replicates. Error bars represent standard deviations (SD) (n = 3). An asterisk represents significant difference of enrichment compared to 28°C sample (P<0.05). **: P<0.01, ***: P<0.001.</p