Process of N27 cells clonal purification.

Abstract

<p><b>(A):</b> Phase contrast image of unpurified N27 cells grown at low density. <b>(B):</b> Immunostaining for TH with green fluorescence in unpurified N27 cells. Shown is a cluster of cells exhibiting bright TH-positive staining. Most cells shown in phase contrast have no TH-immunoreactivity. <b>(C):</b> Schematic drawing of the clonal culture procedures for purifying N27-A cells. Cells were plated at low density to form individual colonies which were picked up and screened in 48- and 96-well plates. The positive clones were expanded in 6-well plates and 10-cm dishes. Bar, 20 μm for both <b>A</b> and <b>B</b>.</p

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