Fluorescence optical data in a 3D printed carbonate centerpiece.

Abstract

<p>A centerpiece featuring a 3 mm deep sector-shaped well at the top was used, with filling and venting holes, and an embossed seal. The focal depth of the fluorescence optics was 2.0 mm. (A) Shown are sedimentation profiles acquired with 561 nm excitation for 46 nM mCherry [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0155201#pone.0155201.ref057" target="_blank">57</a>] dissolved in phosphate buffered saline (dots), and best-fit c(s) sedimentation coefficient distribution with adjustments for characteristic signals of fluorescence detection [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0155201#pone.0155201.ref056" target="_blank">56</a>] (solid lines). The plot appended below shows the residuals of the fit. (B) Corresponding sedimentation coefficient distribution showing a main peak at 2.68 S and diffusional boundary broadening corresponding to a species of 26.9 kDa.</p