Upregulation of therapeutic factors in lungs with congenital diaphragmatic hernia (CDH) following prenatal ONO-1301SR treatment.

Abstract

<p>Relative gene expression of <i>Vegf</i> (<b>A</b>), <i>Hgf</i> (<b>B</b>), and <i>Sdf1</i> (<b>C</b>) in the lungs from control (white bars; n = 5), CDH (black bars; n = 5), and CDH + ONO-1301SR-treated (gray bars; n = 5) pups. The average copy number of gene transcripts was normalized to that of the <i>Gapdh</i> gene. The values are expressed as the mean ± SEM. * 0.01 < <i>P</i><0.05, *** <i>P</i> < 0.001 versus control fetuses; # 0.01 < <i>P</i> < 0.05 versus nitrofen-CDH fetuses. Representative immunoblots of VEGF protein levels in fetal lungs from the control, CDH, and CDH + ONO-130-treated pups (<b>D</b>).</p