Preservation of pulmonary arterial structure in fetuses with congenital diaphragmatic hernia (CDH) following prenatal ONO-1301SR treatment.

Abstract

<p>Representative van Gieson-stained lung sections from control (<b>A</b>), CDH (<b>B</b>), and CDH + ONO-1301SR-treated (<b>C</b>) fetuses at 400× magnification. The medial wall thickness (MWT, %) of control (white bar; n = 6), CDH (black bar; n = 6), and CDH + ONO-1301SR-treated (gray bar; n = 6) groups (<b>D</b>). Representative α-smooth muscle actin (α-SMA) and Ki-67 immunostaining in fetal lung sections from control (<b>E</b>), CDH (<b>F</b>), and CDH + ONO-1301SR-treated (<b>G</b>) pups at 400× magnification. The percentage of Ki-67-positive cells relative to the total number of cells within the medial layers of the pulmonary arteries in the lungs from control (white bar; n = 5), CDH (black bar; n = 6), and CDH + ONO-1301SR-treated (gray bar; n = 5) pups (<b>H</b>). The values are expressed as the mean ± SEM. *** <i>P</i> < 0.001 versus control fetuses; ### <i>P</i> < 0.001 versus nitrofen-CDH fetuses.</p