BVA distribution for a hairpin sample undergoing dynamics.

Abstract

<p>The left panel shows the E-S histogram for a single stranded DNA sample (<i>A</i>₃₁-TA, see text), designed to form a transient hairpin in 400mM NaCl. The right panel shows the corresponding BVA plot. Since the transition between hairpin and open structure causes a significant change in FRET efficiency, <i>s</i>_<i>E</i> lies largely above the static standard deviation curve (<i>red curve</i>).</p