<p>HeLa<sup>UPRT</sup> cells were transfected with either control or specific siRNAs and 48 hours post transfection were infected with DENV2, Ad5 or tested for cell viability (C). 40 hours post infection knockdown efficiencies were assessed at the mRNA levels (A), and DENV and Ad5 titers were determined (B). (<b>A</b>) mRNA levels were measured by RT-qPCR and quantified using the 2<sup>-ΔΔCt</sup> approach and normalized to β-actin mRNA levels. (<b>B</b>) DENV and Ad5 were collected at either 40 hpi or 30 hpi, respectively, and titers were determined by plaque assays. Average viral titers for DENV (dark grey) and Ad5 (white) are reported relative to the titer obtained from the control siRNA knockdown (Log10 = 0). Error bars represent ± standard error, p<0.05 (*) are shown. Absolute values for DENV and Ad5 titers are shown in <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0004921#pntd.0004921.s010" target="_blank">S6 Fig</a>. (<b>C</b>) Relative viability of non-infected cells was measured using an MTT assay (Invitrogen) 48 hours following knockdown by the indicated siRNAs.</p
