IFNαβR deletion prior to secondary viral infection does not limit LCMV-specific T cell attrition or the expression of MHC Class I & Qa-1 in vivo.

Abstract

<p>(A) LCMV immune UBC-CreERT2⁺ and UBC-Cre-ERT2^-IFNαβR^fl/fl mice were tamoxifen injected and rechallenged with LCMV or given a sham injection. LCMV-specific (B) CD8⁺ and (C) CD4⁺ T cells were quantified via standard intracellular cytokine staining at the indicated times p.i. (D & E) Qa-1 and (F & G) MHC Class I expression was measured upon D^bGP_33-41⁺ (left column) and D^bNP_396-404⁺ (right column). Shown in panels D & F are representative histograms (gated on D^bGP_33-41⁺ CD8⁺ T cells). Data in B, C, E, & G are summations of 2 independent experiments (n = 4–7). Significance was determined via two-way ANOVA with Sidak correction (**** p<0.0001, *** p<0.001, ** p<0.01, * p<0.05).</p