Co-localization and focal enrichment of polycomb proteins with LANA in KSHV-infected cells.

Abstract

<p>KSHV-infected iSLK cells (iSLKBAC16-3xF-LANA) were subjected to confocal microscopic analysis for LANA (Green, false color) and polycomb proteins (Red). SPT5 and CYCT1 served as controls. FLAG antibody was used to detect the 3xF-LANA. Representative LANA puncta were connected by white lines and the co-localization of LANA with the indicated cellular proteins was measured by the image processing program ImageJ.</p