Additional LANA expression is not sufficient for the early recruitment of EZH2 onto the KSHV genome.

Abstract

<p><b>(A)</b> Time course analysis of LANA protein expression during <i>de novo</i> infection. <b>(B)</b> Time course ChIP analysis of LANA-binding to viral promoters during <i>de novo</i> infection. <b>(C)</b> Immunoblot analysis of LANA expression in empty lentiviral vector and 3xFLAG-LANA expressing lentivirus infected cells. <b>(D)</b> ChIP assays for binding of LANA and EZH2 to viral promoters in LANA-overexpressing cells during <i>de novo</i> KSHV infection.</p