<p>Number and density of Brn3a+ RGCs in normal embryonic (E8—E20) and post-hatch (P4, P11) retinas. Brn3a+ RGCs were counted using automated cell counting in four retinal quadrants of flat-mount retina. (A) Retinal quadrants, (B) retinal halves (dorsal and ventral) and (A) whole retina from the different ages were compared. (C) Schematic diagram of flat-mount right (RR) and left (LR) retina with quadrants depicted. (D) The total area of flat-mounted retina plotted against the Brn3a+ RGC density (total Brn3a+ RGCs/retinal area) for each age. (E) Representative fluorescence micrographs of Brn3a+ cells in central and peripheral E10, E14, P4 and P11 retinas. One way ANOVA and Tukey’s post-hoc test, * = p<0.05, ** = p< 0.01, *** = p<0.001; £ = p<0.05; ££ = p< 0.01 and £££ = p<0.001, for retinal mean density. Cell counts are listed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0161862#pone.0161862.t001" target="_blank">Table 1</a>. D-T; dorso-temporal, D-N; dorso-nasal, V-N; ventro-nasal, V-T; ventro-temporal.</p

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