The fraction of Brn3a+ cells in the ganglion cell layer of E18 retina.


<p>Representative fluorescence micrograph image sample of the ganglion cell layer (GCL) from E18 chicken retina with (A) Brn3a immunoreactivity and (B) DAPI nuclear staining. Red arrows indicate Brn3a, DAPI double positive cells. Yellow arrows indicate DAPI+, Brn3a negative cells. (C) Fraction of Brn3a+ cells of DAPI+ cells in the GCL. The number of Brn3a+ cells and DAPI+ cells was counted manually in 18 evenly distributed sample areas of an E18 flat-mount retina. Cell counts are listed in <a href="" target="_blank">S1 Table</a> in supporting file.</p

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