Cdc6 contributes to PTX-induced mitotic slippage by inactivation of Cdk1.

Abstract

<p>(A) HepG2 cells were transfected with siRNA targeting cdc6 mRNA for 24h, the inhibitory effect was examined by Western Blotting. (B) HepG2 cells were treated with PTX (30 nM) for 4 and 7 days, NCTD (30 μM) was added to replace PTX after 4 days treatment. Cdc6 RNAi was transfected after PTX treatment for 4 days. Cdc6, pCdk1 and Rad 21 were examined by Western Blotting. GAPDH was used as loading control. The protein levels are expressed as optical density fold difference related to GAPDH (relative OD). Three independent experiments were performed, *<i>P</i><0.05 as compared to PTX group.</p