Cdc6 depletion inhibits mitotic exit in synchronized cells.
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Abstract
<p>HepG2 cells were synchronized by double thymidine block, and then released into fresh medium containing PTX (30 nM) or PTX (30nM) + NCTD (30 μM). For Cdc6 RNAi, the transfection was performed after first thymidine block. Cell cycle profiles were analyzed by FACS after released for indicative time. G2/M (left) and G1 (right) percentage are presented in below panel. Three independent experiments were performed.</p