K_D determination of SMAC-rhodamine binding to BIR2 and BIR3 domains of cIAP1 and cIAP2.

Abstract

<p>Data were for assay conditions consisting of 25 mM Hepes @ 7.5, 1 mM TCEP, 20 nM SMAC-rhodamine with varying concentrations of various BIR domains. For cIAP1-BIR3, assays included 40 mM β-glycerol phosphate. Plates were read on the Analyst and observed mP were plotted against the log of protein concentration.</p