Comparable transcript levels of mSCTR and mAVPR2 in CHOK1 cells transiently transfected with combinations of mSCTR and mAVPR2 by quantitative real-time PCR.

Abstract

<p>The total receptor gene transcript levels were compared to the internal house-keeping control GAPDH by the 2 ΔΔ ct method. There is no significant difference among the groups. mSCTR/pcDNA3.1, 1ug mSCTR plasmid with 1ug pcDNA 3.1 empty vector; mSCTR/mAVPR2, 1ug receptor plasmid each; pcDNA3.1, 2ug pcDNA3.1; mAVPR2/pcDNA3.1, 1ug mAVPR2 plasmid with 1ug pcDNA 3.1. Data are presented as means ± SEM from three independent experiments in duplicate. ns, not significant.</p

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