Preliminary biophysical characterisation of recombinant NLRP1(227–990).

Abstract

<p>(A) Size exclusion chromatography profile of the construct NLRP1(227–990) on a S200 XK16/60 column. Black arrow heads indicate the retention volumes of molecular weight standards. The sample migrates as a single species with an apparent molecular weight between 44–158 kDa. (B) Far-UV circular dichroism spectrum of NLRP1(227–990). (C) Thermal unfolding of NLRP1(227–990) obtained by following the CD signal at 222 nm as a function of the temperature increased at 2°C/min, the value of the mid-point transition is 54.3±0.5°C.</p