DCL levels upon PSTVd infection.

Abstract

<p>(A) Volcano plot from microarray experiment with RNA from leaves of WT and PSTVd <i>N</i>. <i>benthamiana</i> infected plants (3wpi). <i>N</i>. <i>benthamiana</i> genes with no significant alteration of their expression level are indicated with black dots, while red dots represent genes with a significant higher or lower expression (fold change (FC) ≥ 2, Benjamini-Hochberg (BH) FDR-corrected P-value < 0.05) in PSTVd infected plants compared to WT plants. The 35 <i>N</i>. <i>benthamiana</i> RNAi components (described in [<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1005936#ppat.1005936.ref010" target="_blank">10</a>]) are indicated with green dots. The PSTVd virus sequence and its reverse complement (RC) are both labeled (see micorarray design) (B) qPCR experiments of DCL transcripts upon infection. Two reference genes (L23, FBOX) were used for normalization. No significant differences were observed.</p