Expression of RNA aptamers in HUVECs.

Abstract

<p>(<b>A</b>) Total RNA was isolated from transfected (+) and non-transfected (-) cells, then RT-PCR analysis were performed. Expression of the aptamer, PAI-1, and β-actin is shown. N.B. The SM20 was assay was run separately and then added to the figure. (<b>B</b>) HUVECs transfected with aptamers (Sel2, SM20, and WT15) or non-transfected cells were added to vitronectin coated plates and incubated for 1 hour at 37°C. The non-adherent cells were removed and the adherent cells were assessed by an MTT assay analysis. The percent of adherent cells were normalized to the percent of cells adhering in the absence of aptamers. All reactions were done in triplicates and repeated at least twice times; error bars represent the standard deviation of the data. *p<0.05.</p