<p>(A) Immunoblots of indicated proteins in SUM-149 or MDA-MB-231 cells treated with the indicated concentrations of UNC1999 (left panel) or DZNep (right panel) for 72 hours. β-actin serves as a loading control. (B) Immunoblots of indicated proteins in SUM-149 or MDA-MB-231 cells expressing control siRNA or siRNA targeting EZH2, SUZ12 or both EZH2 and SUZ12. β-actin serves as a loading control. (C) ChIP analysis of EZH2 and SUZ12 binding at the RelB promoter or the MYT1 Promoter as a positive control in SUM-149 and MDA-MB-231 cells. Non-specific binding was estimated from IgG immunoprecipitates. Values given as means ± standard deviation from three technical repeats of one representative experiment from the three biological repeats.</p
