<p>(A) Top Panel, Quantification of tumorspheres formed by 100 MCF7, HCC-1428 or T47D cells expressing control siRNA or siRNA targeting EZH2 or RelB. Values given as means ± standard deviation from three biological repeats. *P<0.05 versus controls; two tailed unpaired t-test. Bottom Panel, Immunoblots of indicated proteins in adherent MCF7, HCC-1428 or T47D cells expressing indicted siRNA construct. β-actin serves as a loading control. (B) Left panel, quantification of tumorpsheres formed by 100 MCF7, HCC-1428 or T47D cells treated daily for 3 days with 1μM UNC1999. Values given as means ± standard deviation from three biological repeats. Right panel, immunoblots of the indicated proteins in adherent MCF7, HCC-1428 or T47D cells treated daily for 3 days with 1μM UNC1999 to validate EZH2 inhibition. β-actin serves as a loading control.</p
