Sensitive Monitoring of Fluoroquinolones in Milk and
Honey Using Multiple Monolithic Fiber Solid-Phase Microextraction
Coupled to Liquid Chromatography Tandem Mass Spectrometry
In the present study,
a new multiple monolithic fiber solid-phase
microextraction (MMF-SPME) based on poly(apronal-<i>co</i>-divinylbenzene/ethylenedimethacrylate) monolith (APDE) was synthesized.
The effect of the preparation parameters of APED on extraction efficiency
was studied thoroughly. The combination of APDE/MMF-SPME with high-performance
liquid chromatography tandem mass spectrometry detection (HPLC/MS-MS)
was developed for sensitive monitoring of ultratrace fluoroquinolones
(FQs) in foodstuffs, including milk and honey samples. Under the optimized
experimental conditions, the limits of detection (S/N = 3) for the
targeted FQs ranged from 0.0019 to 0.018 μg/kg in milk and 0.0010
to 0.0028 μg/kg in honey. The relative standard deviations (RSDs)
for method reproducibility were less than 9% in all samples. The established
method was successfully applied for the monitoring of FQs residues
in milk and honey samples with the recoveries between 74.5% and 116%
(RSDs were in the range 0.9–9.5%). In comparison to previous
methods, the developed APDE/MMF-SPME-HPLC/MS-MS showed some merits,
including satisfactory sensitivity, simplicity, high cost-effectiveness,
and low consumption of organic solvent
