Inflammation induced by <i>F</i>. <i>nucleatum</i> is dependent on ROS.
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Abstract
<p>(A) Caco-2 cells were infected by <i>F</i>. <i>nucleatum</i> for the indicated periods of time (3, 6, 12, 24 hours). ROS generation was detected by DCFH-DA assay. (B, C and D) Following pretreatment with 1 mM Tiron or 10 mM N-acetyl-cysteine (NAC) for 6 hours, Caco-2 cells were infected with <i>F</i>. <i>nucleatum</i> (MOI = 100:1) for 12 hours. Supernatants of medium was assessed by ELISA for levels of IL-8, IL-1β and TNF-α. The data are presented as the means ±SEM of at least 3 independent experiments. *, <i>P</i><0.05.</p