H₂O₂ production measured using PO1 fluorescence in control and IP hearts during ischemia / reperfusion.

Abstract

<p><b>A</b>, Averaged fluorescence data for control (black) or IP (grey) hearts perfused with 5 μmol/L PO1. PO1 was present in the perfusion solution from 30 min (Control) or 40 min (IP) before starting global ischemia. PO1 was excluded from the perfusion solution after 30 min of reperfusion. The fluorescence was normalized using the value obtained at -11 min for control and -21 min for IP hearts and then averaged for each group. <b>B</b>, Mean data (± SEM as error bars; n = 11 for control and n = 9 for IP hearts) for PO1 fluorescence during the reperfusion period. <b>C,</b> Mean data (± SEM as error bars; n = 17 for control and n = 16 for CsA-treated hearts) for PO1 fluorescence during reperfusion of control hearts (black) and CsA-treated hearts (grey). Data were normalized to the fluorescence value at -16 min. Where present, CsA (0.2 mmol/L) was added to the PO1 perfusion solution 15 min before ischemia and during 30 min of reperfusion. Statistically significant effects of IP and CsA are shown by the horizontal lines (* p ≤ 0.05; ** p ≤ 0.01). Corresponding infarct sizes are presented in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0167300#pone.0167300.t001" target="_blank">Table 1</a>.</p