<i>Legionella</i>-dependent cell death triggered by MTOR suppression is blocked by lipids supplementation.

Abstract

<p><b>(a)</b> Experimental scheme for the results shown in <b>(b-d, f)</b>. <b>(b-c)</b> <i>Myd88</i>^-/- BMMs with condensed nuclei <b>(b)</b> or positive for phospho-rS6p (MFI>300) <b>(c)</b> produced by infections with <i>ΔflaA</i> (MOI = 20) and the indicated treatments. Infected and neighboring uninfected cells were quantified for each category. <b>(d)</b> Infected and neighboring uninfected BMMs with condensed nuclei after infections with <i>ΔflaA</i> or <i>ΔdotA</i> (MOI = 20) in the presence/absence of FBS. <b>(e)</b> Kinetics of LDH release by C57BL/6 BMMs infected as indicated (MOI = 10) in the presence of FBS or dFBS. <b>(f)</b> Infected <i>Myd88</i>^-/- BMMs with condensed nuclei produced by <i>ΔflaA</i> infection (MOI = 20) and the indicated treatments. <b>(b-f)</b> PP242 (2.5μM), LY294002 (10μM), FBS (10%), dFBS (10%), human LDL (10mg/ml). <b>(b-f)</b> Means ± s.d of technical triplicates for each condition are shown. At least 50 cells <b>(c,f)</b> or 100 cells <b>(b,d)</b> were analyzed for each condition. A representative of two <b>(e-f)</b> or three <b>(b-d)</b> biological replicates is shown for each experiment. <b>(b-f)</b> n.s—not significant, ** p<0.005 (unpaired T-test).</p