<p>(A) Schematic representation of mitochondrial fusion, fission and biogenesis events. Biogenesis is represented by frequency (B<sub>f</sub>) and probability (B<sub>p</sub>) parameters, and can occur in all mitochondria with a mass less than M<sub>max</sub> = 3.0 μm<sup>2</sup>. (B) Basic flow chart of the model representing fusion, fission and biogenesis events. Shaded boxes indicate the main processes of the model (fusion, fission and biogenesis) with governing equation of the biogenesis process. Time step used in all the simulation, Δt = 1 sec. (C) Maximization of the fitness function O<sub>B</sub> within 100 iterations, reaching a value of -0.049, using a classic genetic algorithm (settings described in the Methods section). (D) Mean of 100 simulations of mitochondrial mass growth over 48 hours, beginning with a mitochondrial mass of 1 and using the parameter values obtained from the optimization (C), biogenesis probability = 22.1% and biogenesis frequency = 28.9 minutes.</p
