BDNF promotes activation of Akt signals in MC3T3-E1 cells.

Abstract

<p>Western blot analysis (A) and immunocytochemical staining of Akt and pAkt (Ser473) (B) in MC3T3-E1 cells. (A) BDNF significantly increased the active form of Akt (pAkt) in MC3T3-E1 cells at 30 min. On the other hand, the phosphorylation of ERK was not affected by BDNF. (B) BDNF enhanced pAkt immunoreactivity in the cytoplasm. The nucleus is stained with DAPI (blue).</p