<p>Immunohistochemical staining of BDNF at day 14 and day 28 (A) and trkB at day 7, day 14 and day 28 (B) after osteotomy. BDNF was deposited within newly formed bone matrices at day 14 and day 28 in the BDNF-treated group (A, arrows). TrkB-positive osteocytes were few in the control group (B). On the other hand, the strongly positive immunoreaction of trkB was observed in the osteoblasts (arrowheads) and osteocytes in bone surface and subsurface area (arrows) at day 7, day 14 and day 28 in the BDNF-treated bones (B). The number of trkB-positive osteocytes in the bone surface area was significantly higher in BDNF group at day 7 (C). Bars, 50 μm.</p
