Expression pattern of the four candidate genes with gene-specific primers and the bean actin gene was used as an internal control for qRT-PCR

Abstract

<p>(A) Expression pattern of the four candidate genes in the leaves of the parental Hongyundou and Jingdou plants without inoculation. The graph's horizontal axis indicates the predicted genes (<i>Phvul</i>.<i>001G243500</i>, <i>Phvul</i>.<i>001G243600</i>, <i>Phvul</i>.<i>001G243700</i> and <i>Phvul</i>.<i>001G243800</i>), and the vertical axis indicates the relative expression levels. (B)-(E) Expression analysis of <i>Phvul</i>.<i>001G243500</i>, <i>Phvul</i>.<i>001G243600</i>, <i>Phvul</i>.<i>001G243700</i> and <i>Phvul</i>.<i>001G243800</i>, respectively, in the leaves of Hongyundou and Jingdou at different times (0, 6, 12, 24, 48, 72, 96 and 120 hours after inoculation (HAI) with <i>Colletotrichum lindemuthianum</i> race 81). Plot with the time after inoculation on the horizontal axis and the relative expression level on the vertical axis. The filled red bars in (A), (B), (C), (D) and (E) refers to Hongyundou, and the blue bars refer to Jingdou. The means were generated from three independent measurements, and the bars indicate the standard errors.</p