Kinetics of the circuit. (A) Kinetics of the circuit responding to blue light illumination with constitutive rtTAm expression. The cells were illuminated by blue LED (1.25 W m−2) for 0 h, 5 h, 10 h, 20 h and 40 h, respectively. The data are presented as mean ± SD (n = 6). (B) Kinetics of the circuit responding to Dox with constitutive rtTAm expression. The cells were treated with 1 μg/ml of Dox for 0 h, 5 h, 10 h and 20 h, respectively. The data are presented as mean ± SD (n = 6). (C) Kinetics of the circuit responding to cumate with constitutive mCherry-TCP expression. The cells were treated with 30 μg/ml of cumate for 0 h, 5 h, 10 h, 20 h and 40 h, respectively. The data are presented as mean ± SD (n = 6). (TIF 1013 kb