TCP fusion proteins induce rtTAm-dependent expression of GFP. We transfected HEK cells with mCherry, mCherry-TCP, or mCherry-NLS-TCP constitutive expression plasmids, respectively, also transfected TRE3G promoter controlled GFP expression plasmid to all the three groups of cells. We divided mCherry transfected cells into two dishes and treated one dish of the cells with 1 μg/ml Dox at 24 h after transfection. The images were collected at 72 h after transfection. (TIF 532 kb