Specificity of the mRT-PCR assay.

Abstract

<p>Lane M: molecular marker. Lane 1: a mixture containing equine-origin H3N8 (A/canine/Colorado/6723-8/2008; 148 bp predicted size), hH3N2 (A/Jiangxi/262/05; 303 bp predicted size), H1N1/2009 CIV (A/canine/Beijing/cau2/2009; 407 bp predicted size), and cH3N2 (A/canine/Beijing/364/2009; 544 bp predicted size) influenza viruses. Lane 2: equine-origin H3N8 CIV (A/canine/Colorado/6723-8/2008). Lane 3: hH3N2 influenza virus (A/Jiangxi/262/2005). Lane 4: H1N1/2009 CIV (A/canine/Beijing/cau2/2009). Lane 5: cH3N2 influenza virus (A/canine/Beijing/364/2009). Lane 6: avian-origin H9N2 influenza virus (A/chicken/Jiangsu/TS/2010). Line 7: avian-origin H5N1 influenza virus (A/chicken/Sheny/0606/2008). Lane 8: CDV (CDV-WZ). Lane 9: CPIV. Lane 10: CAV-2. Lane 11: negative control allantoic fluid.</p