PACAP counteracts ketamine induced mTOR activation.

Abstract

<p>NSCs were plated as single cells and treated with 400 μM alone or with 100 nM PACAP and 400 μM ketamine. Cells were treated as indicated and after 1, 2, 6 and 24 hour incubation cells were harvested for Western blot experiments <b>(A)</b>. Cells were treated as indicated and incubated for 1 and 2 hours and harvested for Western blot experiments <b>(B)</b>. To obtain quantitative measurements p-mTOR were normalized against total-mTOR. Data are shown as mean ±SEM (A, n = 4–8; B, n = 3–5). Kruskal-Wallis followed by Dunn’s test or Fisher LSD test was used. Differences were considered significant at P<0.05. * denotes P<0.05 compared with control, # denotes P<0.05 compared to ketamine + 1 hour or ketamine + 2 hours.</p

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