Adherence and invasion of the ΔSdrE and ΔSdrE^278-591 mutants in host cell lines <i>in vitro</i>.

Abstract

<p><i>S</i>. <i>aureus</i> Mu50 and its isogenic mutants <i>ΔSdrE</i> (Mu50Δ<i>SdrE</i>) and ΔSdrE^278-591 (Mu50Δ<i>SdrE-A</i>) were examined for adherence in HeLa (A) and 143B cells (B). These same mutants were also examined for invasivity in HeLa (C) and 143B (D) cells. Infectivity assessments were conducted for 4 h at 37°C. Wild-type <i>S</i>. <i>aureus</i> and the <i>ΔSdrE</i> (Mu50<i>ΔSdrE</i>) and <i>ΔSdrE</i>^278-591(Mu50<i>ΔSdrE-A</i>) mutants were generated to be devoid of SdrE in order to avoid destruction of the monolayer infection system. Scoring of the number of adherent and invasive bacterial cells indicate that adhesion and invasion are substantially reduced for <i>ΔSdrE</i> ((Mu50<i>ΔSdrE</i>) and <i>ΔSdrE</i>^278-591 -deficient (Mu50<i>ΔSdrE-A</i>) <i>S</i>. <i>aureus</i> mutant. Results are presented as a mean ± standard deviation for at least three independent experiments. Asterisks and triangles denote values significantly different from the wild-type as determined by Student’s t-test (** P < 0.01).</p