<p>(A) Western blot analysis was performed in cells treated with dinaciclib (25 nM) or vehicle for the indicated time. The levels of Mcl-1, Bcl-x<sub>L</sub> and survivin were evaluated in BHP7-13, WRO82-1 and 8505C cells. (B) Caspase-3 activity was detected using fluorometric assay kit in cells treated with dinaciclib (25 nM) or vehicle for 24 h. (C) Statistical analyses of early apoptotic cells determined by flow cytometry to detect Annexin V-positive and PI-negative staining showed dinaciclib significantly induced early apoptosis at 24 h in BHP7-13, WRO82-1 and 8505C cells. (D) Sub-G1 apoptosis was detected by measuring the DNA content using flow cytometry in cells treated with dinaciclib (25 nM) or vehicle for 72 h. Dinaciclib increased the proportions of sub-G1 cells in all seven thyroid cancer cell lines. ** <i>P</i> < 0.005 compared with vehicle-treated cells.</p
