<p>(A) RT-PCR and Western Blot analysis of the pPy-Rbm46-E14 stable cell lines. (B) CCK-8 analysis of the proliferation rate of pPE-E14 cells and pPy-Rbm46 cells within 72 hours after seeding. Experiment was performed in triplicate. Error bars showed the standard deviation. (C) Statistics analysis of the average diameter of the colonies formed by pP-E14 cells and pPy-Rbm46 cells, respectively. Experiment was performed in triplicate. Error bars showed the standard deviation. ***, p<0.001; t-test. (D) Representative profiles of the pPE-E14 cells and pPy-Rbm46 cells by Annexin V/PI staining. (E) and (F) RT-PCR analysis of <i>Oct4</i> and <i>Rbm46</i> mRNA level at the indicated time points in E14 cells induced to undergo differentiation by removal of LIF. Experiments were performed in triplicate. Error bars showed the standard deviation.</p
