<p>Siderophore-producing rhizobacteria beneficially affect plant growth by providing available iron to plants. In this study, bacteria were isolated from the rhizosphere of canola (<i>Brassica napus</i> L.) plants grown in the central fields in Iran, for the presence of siderophore-producing bacteria. A total of 45 distinct isolates were found to produce siderophore using qualitative chrome azurol sulfonate (CAS)-agar assay. Of them, ten isolates, based on the highest halo diameter/colony diameter ratios, were selected to quantify the rate of siderophore production using CAS-liquid assay. A variety of biochemical assays was used to determine the type(s) of siderophores produced by each of the ten isolates. The best isolates, based on production of the highest rates of either hydroxamates or carboxylates, were identified and used in further studies. Based on 16S ribosomal ribonucleic acid (rRNA) sequence analysis and a variety of phenotypic properties, the isolates were identified as <i>Micrococcus yunnanensis</i> YIM 65004 (T) and <i>Stenotrophomonas chelatiphaga</i> LPM-5 (T). We also studied the plant growth-promoting effect of the most promising isolates (YIM 65004 and LPM-5) on canola and maize plants under greenhouse conditions. The results of this study showed that <i>M. yunnanensis</i> and <i>S. chelatiphaga</i> increased gain weight and iron (Fe) content of roots and shoots significantly, in comparison with control, indicating beneficial effects of these rhizobacteria on plant growth and development. This study reports <i>M. yunnanensis</i> and <i>S. chelatiphaga</i>, as new records for Iran. The latter is reported for the first time from plant (canola) rhizosphere. Besides, the ability of both <i>M. yunnanensis</i> and <i>S. chelatiphaga</i> to produce siderophores is documented for the first time.</p
