Low expression of miR-532/miR-3064 is associated with poor survival of patients with OC.

Abstract

<p>(<b>A</b>) A schematic diagram explains base pairing between miR-532/3064 with the 3'-UTR sequences of hTERT; (<b>B, C</b>) qPCR analysis of miR-532 (<b>B</b>)/miR-3064 (<b>C</b>) levels in 60 OC patient samples and 20 normal ovarian tissue samples; (<b>D, E</b>) Endogenous expression of miR-532 (<b>D</b>)/miR-3064 (<b>E)</b> was determined using qPCRs in OC cell lines (SKOV-3 and ES-2) and normal ovarian epithelial NOEC cells; (<b>F, G</b>) OC patients were categorized into two groups with high (above median, n = 30) or low (below median, n = 30) miRNA expression. Kaplan-Meier survival curves for OC patients were plotted based on high or low miR-532 (<b>F</b>)/miR-3064 (<b>G</b>) expression; (<b>H</b>) Western blot analysis for the expression of hTERT or GAPDH in SKOV-3, ES-2 and NOEC cells. **: <i>P</i> < 0.01.</p