Bioinformatics analyses of <i>OsMTP11</i> nucleotide and amino acid sequences.

Abstract

<p><b>A.</b><i>OsMTP11</i> gene structure analysis in the Rice Genome Annotation Database (<a href="http://rice.plantbiology.msu.edu/" target="_blank">http://rice.plantbiology.msu.edu/</a>). <b>B.</b> Phylogenetic tree of the <i>MTP</i> family from rice and <i>Arabidopsis</i>. The tree was constructed using MEGA 4.0.2 by the neighbor-joining method. <i>Arabidopsis</i> MTP amino acid sequences were obtained from <a href="http://www.tigr.org" target="_blank">www.tigr.org</a>: AtMTP1, At2g46800; AtMTP2, At3g61940; AtMTP3, At3g58810; AtMTP4, At2g29410; AtMTP5, At3g12100; AtMTP6, At2g47830; AtMTP7, At1g51610; AtMTP8, At3g58060; AtMTP9, At1g79520; AtMTP10, At1g16310; AtMTP11, At2g39450; AtMTP12, At2g04620. Rice MTP amino acid sequences were downloaded from <a href="http://rice.plantbiology.msu.edu/" target="_blank">http://rice.plantbiology.msu.edu/</a>. OsMTP11, Os01g62070; OsMTP1, Os05g03780; OsMTP5, Os02g58580; OsMTP6, Os03g22550; OsMTP7, Os04g23180; OsMTP8, Os02g53490; OsMTP8.1, Os03g12580; OsMTP9, Os01g03914; OsMTP11, Os01g62070; OsMTP11.1, Os05g38670; OsMTP12, Os08g32680. <b>C.</b> Amino acid alignment of OsMTP11, AtMTP11 [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0174987#pone.0174987.ref011" target="_blank">11</a>] and ShMTP1 (AY181256) [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0174987#pone.0174987.ref012" target="_blank">12</a>]. Amino acid sequences of four predicted transmembrane (TM) segments are boxed. Amino acid residues with dark shading indicate conserved sequences, and residues with light gray shading indicate those conserved in two protein sequences. <b>D.</b> The predicted transmembrane helices of OsMTP11. The transmembrane domains were estimated using TMHMM2: <a href="http://www.cbs.dtu.dk/services/TMHMM/" target="_blank">www.cbs.dtu.dk/services/TMHMM/</a>. The peaks show the predicted transmembrane (TM) regions of proteins. These data indicate that OsMTP11 has four obvious TM regions.</p

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