El propósito es describir las características socio demográficas, las condiciones de trabajo y situación de salud de los trabajadores para aportar información y conocer la magnitud y trascendencia de esta problemática y tomar decisiones necesarias de prevención

Yachun Su

Yuye Zhang

Ning Huang

Feng Liu

Weihua Su

Liping Xu

Waqar Ahmad

Qibin Wu

Jinlong Guo

Youxiong Que

BMC Genomics

Abstract Background Sugarcane smut caused by Sporisorium scitamineum leads to a significant reduction in cane yield and sucrose content. MicroRNAs (miRNAs) play an important role in regulating plant responses to biotic stress. The present study was the first to use two sugarcane genotypes, YA05-179 (smut-resistant) and ROC22 (smut-susceptible), to identify differentially expressed miRNAs in sugarcane challenged with S. scitamineum by using high-throughput sequencing. Results The predicted target gene number corresponding to known differentially expressed miRNAs in YA05-179 was less than that in ROC22, however most of them were in common. Expression of differential miRNAs under S. scitamineum challenge was mostly downregulated, with similar trends in the two varieties. Gene ontology (GO) analysis showed that the target gene classification of known miRNAs was similar to that of the newly identified miRNAs. These were mainly associated with cellular processes and metabolic processes in the biological process category, as well as combination and catalytic activity in the molecular function category. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis revealed that these predicted target genes involved in a series of physiological and biochemical pathways or disease resistance-related physiological metabolism and signal transduction pathways, suggesting that the molecular interaction mechanism between sugarcane and S. scitamineum was a complex network system. These findings also showed certain predicted target genes of miR5671, miR5054, miR5783, miR5221, and miR6478 play roles in the mitogen-activated protein kinase (MAPK) signaling pathway, plant hormone signal transduction, and plant-pathogen interaction. Quantitative real-time PCR (qRT-PCR) analysis showed that majority of the known miRNAs and its predicted target genes followed a negatively regulated mode. Seven out of eight predicted target genes showed identical expression after 12 h treatment and reached the highest degree of matching at 48 h, indicating that the regulatory role of miRNAs on the target genes in sugarcane was maximized at 48 h after S. scitamineum challenge. Conclusions Taken together, our findings serve as evidence for the association of miRNA expression with the molecular mechanism underlying the pathogenesis of sugarcane smut, particularly on the significance of miRNA levels in relation to the cultivation of smut-resistant sugarcane varieties

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Small RNA sequencing reveals a role for sugarcane miRNAs and their targets in response to Sporisorium scitamineum infection

Hernández Mendoza, Sonia Zulema

Pérez Coto, Ruby Lorena

Repositorio Institucional UNAN-Managua

Condiciones Laborales y de Salud de Los Trabajadores de la Maquila del Tabaco. Ciudad El Paraíso, Honduras. Octubre 2003 a Marzo 2004.

http://repositorio.unan.edu.ni/5535/1/t245.pdf

Condiciones Laborales y de Salud de Los Trabajadores de la Maquila del Tabaco. Ciudad El Paraíso, Honduras. Octubre 2003 a Marzo 2004.

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Repositorio Institucional UNAN-Managua