MICs of evolved strains obtained under single drug application. The MICs to (a) AMK, (b) ENX, and (c) CP of the parent strain (P) and strains evolved under single drug application (AMK, ENX, and CP) are presented. Evolved strains were obtained by isolating a single clone from the end-point culture of the laboratory evolution, and used to quantify MICs. (PDF 376 kb